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anti sheep cd41 61  (Bio-Rad)


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    Bio-Rad anti sheep cd41 61
    Anti Sheep Cd41 61, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+sheep+cd41+61/pm41789552-70-10-19?v=Bio-Rad
    Average 93 stars, based on 12 article reviews
    anti sheep cd41 61 - by Bioz Stars, 2026-07
    93/100 stars

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    Cytometric gating protocol. A. Platelet gate (P1) on the basis of the forward (FSC) and side scatter (SSC), threshold FSC 20,000. B. Single-parameter fluorescence histogram of the APC-conjugated polyclonal goat anti-mouse antibody as isotype-negative control for <t>CD41/61</t> (FL-4: 640 nm, filter 675/25 nm). C. Single-parameter fluorescence histogram gated for APC-conjugated monoclonal mouse anti-sheep antibodies against CD41/61. D. Dotplot representing platelet expression of CD41/61 ( y -axis; FL-4; Q1-UL indicating positive events for CD41/61) and expression of CD11a/18 (leukocytes; x -axis; FL-1, 488 nm, filter 530/30 nm; Q1-LR indicating positive events for CD 11a/18); dual-labeled events positive for CD41/61 and CD11a/18 representing 3.5% platelet-leukocyte aggregates in Q1-UR.
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    Cytometric gating protocol. A. Platelet gate (P1) on the basis of the forward (FSC) and side scatter (SSC), threshold FSC 20,000. B. Single-parameter fluorescence histogram of the APC-conjugated polyclonal goat anti-mouse antibody as isotype-negative control for <t>CD41/61</t> (FL-4: 640 nm, filter 675/25 nm). C. Single-parameter fluorescence histogram gated for APC-conjugated monoclonal mouse anti-sheep antibodies against CD41/61. D. Dotplot representing platelet expression of CD41/61 ( y -axis; FL-4; Q1-UL indicating positive events for CD41/61) and expression of CD11a/18 (leukocytes; x -axis; FL-1, 488 nm, filter 530/30 nm; Q1-LR indicating positive events for CD 11a/18); dual-labeled events positive for CD41/61 and CD11a/18 representing 3.5% platelet-leukocyte aggregates in Q1-UR.
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    Bio-Rad monoclonal mouse anti sheep cd41 61
    Cytometric gating protocol. A. Platelet gate (P1) on the basis of the forward (FSC) and side scatter (SSC), threshold FSC 20,000. B. Single-parameter fluorescence histogram of the APC-conjugated polyclonal goat anti-mouse antibody as isotype-negative control for <t>CD41/61</t> (FL-4: 640 nm, filter 675/25 nm). C. Single-parameter fluorescence histogram gated for APC-conjugated monoclonal mouse anti-sheep antibodies against CD41/61. D. Dotplot representing platelet expression of CD41/61 ( y -axis; FL-4; Q1-UL indicating positive events for CD41/61) and expression of CD11a/18 (leukocytes; x -axis; FL-1, 488 nm, filter 530/30 nm; Q1-LR indicating positive events for CD 11a/18); dual-labeled events positive for CD41/61 and CD11a/18 representing 3.5% platelet-leukocyte aggregates in Q1-UR.
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    Cytometric gating protocol. A. Platelet gate (P1) on the basis of the forward (FSC) and side scatter (SSC), threshold FSC 20,000. B. Single-parameter fluorescence histogram of the APC-conjugated polyclonal goat anti-mouse antibody as isotype-negative control for CD41/61 (FL-4: 640 nm, filter 675/25 nm). C. Single-parameter fluorescence histogram gated for APC-conjugated monoclonal mouse anti-sheep antibodies against CD41/61. D. Dotplot representing platelet expression of CD41/61 ( y -axis; FL-4; Q1-UL indicating positive events for CD41/61) and expression of CD11a/18 (leukocytes; x -axis; FL-1, 488 nm, filter 530/30 nm; Q1-LR indicating positive events for CD 11a/18); dual-labeled events positive for CD41/61 and CD11a/18 representing 3.5% platelet-leukocyte aggregates in Q1-UR.

    Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

    Article Title: Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome

    doi: 10.1177/10406387221077969

    Figure Lengend Snippet: Cytometric gating protocol. A. Platelet gate (P1) on the basis of the forward (FSC) and side scatter (SSC), threshold FSC 20,000. B. Single-parameter fluorescence histogram of the APC-conjugated polyclonal goat anti-mouse antibody as isotype-negative control for CD41/61 (FL-4: 640 nm, filter 675/25 nm). C. Single-parameter fluorescence histogram gated for APC-conjugated monoclonal mouse anti-sheep antibodies against CD41/61. D. Dotplot representing platelet expression of CD41/61 ( y -axis; FL-4; Q1-UL indicating positive events for CD41/61) and expression of CD11a/18 (leukocytes; x -axis; FL-1, 488 nm, filter 530/30 nm; Q1-LR indicating positive events for CD 11a/18); dual-labeled events positive for CD41/61 and CD11a/18 representing 3.5% platelet-leukocyte aggregates in Q1-UR.

    Article Snippet: Activated platelets and PLAs were measured by dual-labeling with conjugated antibodies (all Bio-Rad, formerly AbD Serotec) as described., An allophycocyanin (APC)-conjugated (LYNX rapid antibody conjugation kit; Bio-Rad) monoclonal mouse anti-sheep antibody against CD41/61 with cross-reactivity against equine platelet CD41/61 , , was used as platelet marker (MCA1095GA, dilution 1:50 with modified HEPES/Tyrod buffer ).

    Techniques: Fluorescence, Negative Control, Expressing, Labeling